Effects of Binary Mixture of Insecticides on Hematology and Histopathology of Liver in Wistar Albino Rats
Keywords:
HEMATOLOGY AND HISTOPATHOLOGY, EFFECTS OF BINARY MIXTURE OF INSECTICIDES, ALBINO RATS, HISTOPATHOLOGY OF LIVER IN WISTAR ALBINO RATSSynopsis
Insecticides are commonly used to control agricultural, home pests and boost crop yields all over the world. Increased use of insecticides is hazardous to non-target organisms and can endanger the health of animals and humans. This research was designed to study the hematological and histopathological alterations in the liver of Wistar albino rats (Rattus norvegicus) in response to Chlorpyrifos (CPF) and Fipronil (FPN). Animal samples were collected from the Animal House, UAF. Rats were acclimatized in the stainless-steel cages for one week before the commencement of the experiment and were treated with two different concentrations of a binary mixture of CPF and FPN (3.1mg/kg + 2mg/kg) and (9.7mg/kg + 6.2mg/kg), respectively. After the completion of experimental trials, rats were slaughtered, and animal samples were taken. Blood samples were taken to estimate the hematological parameters like RBCs, WBCs, hemoglobin, MCH, MCV, MCHC, and PCV. The liver tissues of rats were fixed and stained with hematoxylin-eosin for the diagnosis of histopathological alterations. The results indicated that exposure to the binary mixture of insecticides causes a significant (p˂0.05) decrease in the RBCs count, MCH, MCHC, and hemoglobin level, while on the other hand, it causes a significant (p˂0.05) increase in the WBCs count, MCV, and platelets in the insecticides treated groups. The exposure to insecticides also caused a reduction in the body weight of insecticide-treated groups as compared to the control. The tissue damage confirmed through histopathological examination revealed hepatocytic vacuolation, degeneration of hepatocytes, sinusoidal dilation, and focal necrosis of the degenerated portal vein in the liver of insecticide-exposed rats. It is therefore suggested that necessary precautions should be taken whenever insecticides are used or disposed of in areas with close mammalian proximity.
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